Fig. 6.

Effects of neutrophils on lymphocytes. a, b Changes in expression of MHC-II of neutrophils, which were stimulated with LPS (1 μg/mL) in 0, 2, 4 and 6 h. c Changes in expression of PD-L1 in neutrophils, which were stimulated with LPS (1 μg/mL) in 0, 4, 12, 16, 24 h (n = 3). *P < 0.05 and **P < 0.01, compared with control group (Student’s t test). d–f The effect of neutrophils on count of CD4 and CD8 cells. The neutrophils were stimulated with LPS (1 μg/mL) in advance for 4 h. CD4 and CD8 cells were detected on day one and day four of co-culture. The count change between day one and day four was also observed (n = 3). ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 and ^####p < 0.0001 compared with T group; *p < 0.05, compared with T + N group