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. 2021 Jan 25;118(5):e2012229118. doi: 10.1073/pnas.2012229118

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Fig. 1. — Schematic of multicolor single-molecule imaging enabled by NanoEP delivery. (A) A schematic of the NanoEP device. A nanopore-embedded PC water-filter membrane is sealed at the base of PDMS holder to connect delivery proteins and cell membranes. (B) An SEM image of randomly distributed 100-nm nanopores on the PC membrane. (C) A schematic of multichannel single-molecule imaging of the EGFR signaling pathway. Dye-labeled Grb2 and RBD are delivered into cells, while SOS and Src are expressed by plasmid transfection.