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Design and synthesis of glycan FRET probes. (A) Glycan microarray data showing Ab binding is affected by acetylation of GXM in a concentration-dependent manner (200 μM, 100 µM, 50 µM). Bars represent the mean ± SD. (B) Synthesis of two FRET probes. FRET-OAc 16 bears a 6-O-acetylation pattern, while FRET 17 in contrast bears no acetylation pattern (* = no 6-OAc). Glycans are depicted according to symbol nomenclature for glycans (61).
