Fig. 4.

Recovery of vaginal opening (VO) and occurrence of persistent estrus in the KNDy neuron-rescued female rats. (A) Days at the VO (expressed as a percentage of the total number of animals per group). There were significant differences in the occurrence probability of the VO between the groups (P < 0.05, Kaplan–Meier analysis and log-rank test). (B) Vaginal smear pattern in a representative animal in each group. CC, cornified cells; NC, nucleated cells; and LC, leukocytes. (C) Days that animals showed CC and a mixture of NC and CC (NC/CC) in the first, second, and third weeks after the AAV-Kiss1 injection. Note that no animal showed a LC smear pattern. N/A, not applicable because animals failed to show VO.