Fig. 4.

Liver-specific miR-33 deficiency reduces the expression of genes related to hepatic fibrosis and inflammation. RNA-seq from livers of male miR-33^loxP/loxP (WT) and miR-33^loxP/loxP/Albumin^Cre (LKO) mice fed an HFD. (A) Principal component analysis of RNA-seq data from WT and LKO mice. (B) Heat map depicting differentially expressed genes between WT and LKO mice. (C) Top 15 canonical pathways overrepresented among differentially expressed genes in RNA-seq analysis of WT and LKO mice. Orange bars indicate pathways in which genes are consistently up-regulated, blue indicates pathways in which genes are consistently down-regulated, and gray indicates pathways in which some genes are significantly altered in both directions. (D and E) Heat maps depicting differentially expressed genes involved in hepatic fibrosis and hepatic stellate cell activation (D) and leukocyte extravasation (E) pathways.