Table 5.

Strong T-cell responses against regions contained in our designed immunogen are detected by ELISPOT assays in all four immunized animals (data shown are for 50 wk after the prime)

Region	Animal 1	Animal 2	Animal 3	Animal 4
Gag 1 to 52	3,250
Gag 160 to 212	540	1,460	1,750	2,590
Gag 204 to 253		720
Gag 241 to 294			380	400
Env 487 to 540		3,830

The first column lists the HXB2 positions of the regions in the PTE peptide subpools that elicit strong ELISPOT responses (greater than 350 spot-forming cells [SFC] per million peripheral blood mononuclear cells). Columns 2 to 5 list the strength of the response detected in each immunized animal after the last boost. As described in the main text, in assays with pooled peptides, animal 2 also developed a response to a region in Pol that is contained in our immunogen.