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. 2021 Jan 28;22(3):1275. doi: 10.3390/ijms22031275

Figure 3.

Figure 3

Effects of PLEKHA7 re-expression on SUM149 cell growth and survival in 3D culture. (A) SUM149 LZRS ms neo (control) and SUM149 LZRS PLEKHA7 cells were grown in Matrigel for approximately two weeks. Representative images at 4×, 10×, and 20× magnification are shown. Scale bar in each image = 100µm. (B) Quantification of SUM149 LZRS ms neo or SUM149 LZRS PLEKHA7 colonies from 3A are shown as the fold change from SUM149 LZRS ms neo. * indicates p < 0.05, Student’s t-test. (C) Representative images from SUM149 LZRS ms neo and SUM149 LZRS PLEKHA7 suspension cultures undergoing sphere compaction over 18 h. Images taken at 4×. Scale bar in each image = 100µm. (D) A representative graph of SUM149 LZRS ms neo and SUM149 LZRS PLEKHA7 suspension cultures compacting into spheres over 18 h under ultralow attachment conditions. *** indicates p < 0.001, Student’s t-test (p < 0.0001). (E) A representative graph of normalized ATP content produced by SUM149 LZRS ms neo and SUM149 PLEKHA7 spheres after treatment with various concentrations of DOXIL/doxorubicin for 72 h. ** indicates p < 0.01, Student’s t-test (p = 0.002 for 1 µM doxorubicin and 10 µM doxorubicin). Each group was normalized to no treatment.