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. 2021 Jan 22;22(3):1069. doi: 10.3390/ijms22031069

Figure 2.

Figure 2

siRNA against Panx1 increases the migration index in HDF cultures. (A) Representative images of the wound healing process in cultures using Panx1 or Panx3 knockdown cells. HDFs transfected with siRNAs against Panx1, Panx3, or the control plasmid (CTL; siRNA-Empty-GFP) were identified by GFP expression. Dashed lines indicate the wound area (or the migration front line). Bar: 200 µM. Supplementary Figure S1B shows these same fluorescent images superimposed with their respective bright-field images. (B) Quantitative analysis of the average migration index 24 h after transfection of HDFs with respective siRNAs and CTL plasmid. Statistical significance ** p = 0.011; *** p < 0.001; n = 10 experimental replicas from 4 different cultures (one-way ANOVA followed by Tukey’s post hoc test). (C) Representative Western blots of Panx1 and Panx3 expression levels in total lysates from HDFs 12 h post-transfection. Graphs depicting the relative amount of Panx1 and Panx3 normalized to β-Tubulin from 4 different cultures. *** p < 0.001 for siRNA fibroblasts compared to control (unpaired two-tailed t-test). All data are shown as mean ± SEM.