Figure 3.

Activity of Panx1 channels during wound closure in HDFs. (A) Representative fluorescent images of Ethidium (Etd) uptake by HDF cells treated with 200 µM PBN, 100 µM ¹⁰Panx, 100 µM Sc ¹⁰Panx, or vehicle (Control, CTL). Bar: 200 µm. Supplementary Figure S3A shows these same fluorescent images superimposed with their respective bright-field image. (B) Etd uptake rate measure as described in Section 4; *** p < 0.001; n = 10 (two-way ANOVA followed by Bonferroni’s post hoc test). (C) Measurements of extracellular ATP released from HDFs as a function of time in the presence or absence of 200 µM PBN or 10 µM A-740003. #: *** p < 0.001; n = 10 (two-way ANOVA followed by Tukey’s post hoc test). All data are shown as mean ± SEM.