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. 2021 Jan 28;22(3):1297. doi: 10.3390/ijms22031297

Figure 2.

Figure 2

SaARF4 was expressed in the vascular tissues and induced by auxin. (A) The β-glucuronidase (GUS) signals can be detected in the roots, stems, and leaves of ProARF4::GUS. The cross sections (B) and longitudinal sections (C) of stems were made for identifying the expression location of SaARF4. (D) The expression location of SaARF4 in the leaves. (E) The expression location of SaARF4 in longitudinal sections of the leaves. (G) The distribution of GUS signal in ProARF4::GUS roots without any treatment. (H,I) Naphthylphthalamic acid (NPA) (10 μM) and indoleacetic acid (IAA) were used to determine whether the changes in auxin content affected SaARF4 expression in the roots. (F) The expression level of ProARF4::GUS under IAA treatment was detected by qRT-PCR based on three biological replicates and three technical replicates. The red arrows indicate GUS signals. * p < 0.05.