Figure 3.

SaARF4 affected ethylene production and auxin transport. The ethylene production of WT and SaARF4-OE, during 0–5 h under Cd stress is shown in (A). ProDR5:GUS was transformed into the WT and SaARF4-OE lines to identity the auxin distribution model (B). Most of the PINs related to auxin transport in the roots were influenced by the overexpression of SaARF4 (C). Moreover, similar expression modes could be obtained by applying pyrazinamide (PZA) to the WT (D). Under Cd stress (50 μM CdCl₂), the number of adventitious roots (Ars) and lateral roots (LRs) under the influence of PZA were lower than those of the control plants (E,F). These assays were conducted with 100 μM PZA. * p < 0.05; ** p < 0.01.