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. 2021 Jan 26;26(3):640. doi: 10.3390/molecules26030640

Figure 5.

Figure 5

The involvement of FAK in the mechanism underlying the kahweol effect on migration in CTGF-stimulated VSMCs. (A,B) Cells were pretreated with rCTGF (50 ng/mL) for one hour and then treated with kahweol (10 μM) for 24 h. Soluble lysates were subjected to Western blotting for FAK, Erk, and Akt. Protein levels of phosphorylated FAK, Erk, Akt, and β-actin were determined by Western blotting with specific antibodies. Bar graphs present the densitometric quantification of the Western blot bands. (C) Cells were scratched with a micropipette tip to form a cell-free (wounded) area and pretreated with rCTGF (50 ng/mL) for one hour and then treated with kahweol (10 μM) for for 48 h. Wound areas were visualized using a phase-contrast microscope. The distance migrated was determined as the average of the distances of each cell from the wound boundary. Results are representative of three independent experiments. *, p < 0.05 vs. untreated control; #, p < 0.05 vs. rCTGF group.