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. 2021 Jan 25;22(3):1153. doi: 10.3390/ijms22031153

Figure 3.

Figure 3

Effects of ERβ- (PHTTP) or ERα-selective antagonists (MPP) on SRC (Tyr419) expression and phosphorylation in androgen-independent prostate cancer cells PC-3 induced by DPN or PPT. The cells were incubated in the absence (C, control) and presence of DPN (10 nM) for 30 min (A) or PPT (10 nM) for 1 h (B) at 37 °C. The cells were also pre-treated with PHTPP (10 nM) (A) or MPP (10 nM) (B) for 30 min, and then incubated or not, respectively, with DPN for 30 min (A) or PPT for 1 h (B). Phosphorylated SRC (p-SRC) and total SRC were detected by Western Blot. The immunoassay was performed with anti-phosphorylated SRC (upper panel) and anti-total SRC antibodies (lower panel). The relative position of SRC was determined from the molecular weight standard. The data shown are representative of three independent experiments. Densitometric analysis was performed of the results obtained from each band, normalized by the expression of the total SRC and expressed in relation to the control (C = 1). Results were plotted (mean ± SEM) of three independent experiments. * Significantly different from control (C) (p < 0.05, ANOVA and Newman-Keuls). # Significantly different from DPN for 30 min or PPT for 1 h (p < 0.05, ANOVA and Newman-Keuls).