Figure 6.

Effects of the selective inhibitor for SRC-family kinases (PP2) on the expression of non-phosphorylated β-catenin in androgen-independent prostate cancer cells PC-3 induced by DPN. Cells were incubated in the absence (C, control) and in the presence of DPN (10 nM) for 2 h at 37 °C. The cells were also pre-treated with PP2 (5 nM) for 30 min and then incubated or not with the DPN for 2 h. Positive immunostaining for non-phosphorylated β-catenin (green) was detected using the polyclonal antibody produced in rabbit by immunization with the synthetic peptide corresponding to the region around Serine 37 (Ser33/37/Thr41) of human β-catenin. Nuclei were stained with DAPI (blue). Negative control, in the absence of the primary antibody (detail). Results are representative of four independent experiments.