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. 2021 Jan 26;22(3):1204. doi: 10.3390/ijms22031204

Figure 2.

Figure 2

Competitive gel-based ABPP reveals variable MAGL and FAAH activities in rat brainstem and cervical, thoracic and lumbar spinal cord. (A) Brainstem and spinal cord proteomes were incubated for 1 h with vehicle (DMSO), MAGL inhibitors JJKK-048 (100 nM) and KML29 (1 μM) and FAAH inhibitor JZP327A (1 μM), and then labeled with the fluorescent probe TAMRA-FP, as indicated in Materials and Methods. FAAH and MAGL were identified based on selective inhibition and their expected molecular weights. Note that basal MAGL activity was high in the lumbar spinal cord but less intense in samples of the brainstem, cervical and thoracic spinal cord. Based on this analysis, FAAH activity was not found in samples of the brainstem and spinal cord. (B) Histograms comparing the basal activity of MAGL and FAAH in the brainstem and different spinal cord parts. Basal MAGL activity was approximately 2-fold higher compared to that of FAAH in brainstem, and ~4-fold higher in thoracic spinal cord. In the lumbar spinal cord, MAGL activity was ~8-fold higher than of FAAH. Unpaired t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, ns = nonsignificant, n = 11 (BS, cSC) and n = 8 (tSC, lSC).