Figure 2.

Schematic showing reaction of PMDA with lysine on the Affimer and the analyte binding. (a), Affimers were functionalised by conjugating PMDA to the scaffold. The modification forms a peptide bond between carboxylic groups on the PMDA to the Affimer lysines, whilst the other PMDA anhydride becomes hydrolyses, providing two additional carboxy groups. (b), the analyte (human myoglobin) binds to the Affimer variable loops and thereby partially shields the chelated Eu³⁺ from excitation.