Figure 3.

Excitation and emission spectra of Eu³⁺ complex. (a), peak excitation wavelengths for Eu³⁺ complex were measured and using λ_em = 615 nm; (b), peak emission wavelengths for Eu³⁺ complexes were measured for free Eu³⁺ (black line peaks) and Eu³⁺ chelated by PMDA modified anti-human myoglobin Affimer complex (red line peaks). The electronic transition corresponding to the emission peaks are indicated. λ_ex = 395 nm.