Figure 6.

Time-resolved fluorescence counts of Eu³⁺ complex in 1% (v/v) serum. (a), time resolved fluorescence counts of Eu⁺³ chelated by PMDA modified anti-CRP Affimer; (b), time resolved fluorescence counts of Eu⁺³ chelated by PMDA modified anti-CEA Affimer. HFABP3 were used as a control. (c), time resolved fluorescence counts of Eu³⁺ complexes quenching against increased concentration of CRP from 100 fM to 100 nM whereas further quenching was observed following the addition of 100 nM polyclonal anti- CRP IgG. (d), time resolved fluorescence counts of Eu⁺³ chelated by PMDA modified anti-CRP Affimer followed adding of 10 µM EDTA and 1 µM citrate. Some error bars are smaller than data points (*, ** and *** indicate significance with p-value < 0.05, 0.01, and 0.001 respectively), (Data are means ± SEM (n = 3)).