Table 1.

Showing the percentage quench in fluorescence intensity of the chelated Eu³⁺ to PMDA modified human myoglobin, GFAP, CRP and CEA Affimer in 1% (v/v) serum.

Biomarker	Molecular Weight (KDa)	% ∆ Fluorescence Counts of Biomarker	% ∆ Fluorescence Counts of Biomarker + Polyclonal Ab
Myoglobin	17	7.44 ± 1.17	17.25 ± 2.37
GFAP	55	4.66 ± 0.65	12.30 ± 0.46
CRP	119	10.05 ± 2.46	22.08 ± 0.85
CEA	200	17.05 ± 1.50	36.31 ± 6.24

∆ % fluorescence counts of chelated Eu³⁺ by PMDA modified human myoglobin, GFAP, CRP and CEA Affimers were measured at 100 nM concentration of biomarker. Data are means ± SEM (n = 3).