Figure 9.

PAFC tolerance of tomato plant leaves and protective effect of PAFC against B. cinerea infection. The phenotype of leaves is shown in the left panels and damage evaluation with Evan’s blue staining in the right panels. (A) Leaves were either left untreated or treated with 10 µL of 0.1 × PDB (negative controls). (B) For toxicity testing, leaves were treated with 10 µL of PAFC (1 mg mL⁻¹). (C) Plant protection efficacy was evaluated with leaves infected with 10 µL of B. cinerea conidia (10⁷ conidia mL⁻¹) (infection control) and conidia mixed with PAFC (1 mg mL⁻¹). Plants were then further incubated for four days at 23 °C under photoperiodic day/night simulation (12/12 h with or without illumination at 1200 lux) and then harvested for staining. Scale bar, 10 mm.