Table 2.

Estimation of secondary structural components of PAFC from ECD spectra compared to those obtained from NMR measurements.

Secondary Structural Elements/%	PAFC 25 °C	PAFC 95 °C	PAFC 25 °C after Heating	BP Crystallographic Structure (1UOY) §	PAFC Solution NMR Structure (6TRM) §
α- or 310-helix	13	15	14	5	6
β-strand, extended	28	26	28	39	38
β- or γ-turns, bends	26	26	25	31	33
unordered	33	34	32	25	23

^§ The fraction of secondary structural elements was determined by DSSP analysis [36] of the NMR-derived PAFC structure and the crystallographic structure of the homologous P. brevicompactum BP [24].