Table 3.
PAFC inhibitory concentrations that reduce the growth of microorganisms by ≥90% (IC90) $.
| Microorganism | IC90 | |
|---|---|---|
| µM | μg mL−1 | |
| Aspergillus fumigatus | >30 | 199 |
| Aspergillus nidulans | 30 | 199 |
| Aspergillus niger | >30 | 199 |
| Neurospora crassa | 15 | 99 |
| Penicillium chrysogenum | 50 | 331 |
| Microsporum gypseum | 7 | 46 |
| Trichophyton rubrum | 5 | 33 |
| Bacillus subtilis | >50 | 331 |
| Escherichia coli | >50 | 331 |
$ Broth microdilution assays were performed to determine the IC90. Microorganisms were grown in ten-fold-diluted potato dextrose broth (0.1 × PDB) for 24 h at 30 °C in static culture before measurement of the optical density at wavelength 620 nm (OD620nm). Exceptions: N. crassa was grown for 16–30 h at 25 °C, and M. gypseum and T. rubrum were grown for eight days at 30 °C. IC90 was defined as the PAFC concentration that inhibited growth by ≥90% compared to the untreated control, which represented 100% growth.