Table 4.
Physicochemical characteristics and the fungal growth inhibitory potential (IC90) of the peptides PCγ15, PCγ17, and PCγC-terminal compared to the full-length MP PAFC §.
| Protein/Peptide | Molecular Mass (kDa) | Charge (pH 7.0) |
GRAVY | IC90 | |||
|---|---|---|---|---|---|---|---|
| N. crassa | C. albicans | ||||||
| μM | μg mL−1 | μM | μg mL−1 | ||||
| PAFC | 6.6 | +0.6 | −0.767 | 12.5 | 83 | 2.5 | 17 |
| PCγ15 | 1.5 | −0.2 | +0.087 | >100 | 66 | >100 | 66 |
| PCγ17 | 1.8 | +1.1 | −0.367 | 25 | 166 | >100 | 66 |
| PCγC-terminal | 0.7 | +0.8 | +0.038 | >100 | 66 | >100 | 66 |
§ The calculated molecular mass (kDa, ExPasy ProtParam tool), the predicted net charge (at pH 7, http://protcalc.sourceforge.net), and the GRAVY value (www.gravy-calculator.de) are indicated. For determination of IC90 values in 0.1 × PDB, C. albicans was grown for 24 h at 30 °C and N. crassa for 16–30 h at 25 °C. IC90 was defined as a protein/peptide concentration that inhibited growth by ≥90% compared to the untreated control, which represented 100% growth.