Table 2.
Diagnostic performance of nine biomarkers overlapping between PEA and LC/MRM-MS measurements for detecting CRC cases from participants in screening colonoscopy
| Marker | Pearson’s Correlation Coefficient |
PEA Measurements | LC/MRM-MS Measurements | DeLong p-value for AUC Testing |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AUC (95% CI) |
p-value | p-value adj | Se% at 80% Sp | Se% at 90% Sp | AUC (95% CI) |
p-value | p-value adj | Se% at 80% Sp | Se% at 90% Sp | |||
| CDH5 | 0.43 | 0.63 (0.54–0.72) |
<0.01 ** | <0.05 * | 32 | 11 | 0.53 (0.43–0.62) |
0.55 | 0.61 | 21 | 16 | 0.06 |
| IGFBP2 | 0.73 | 0.55 (0.45–0.65) |
0.31 | 0.38 | 30 | 23 | 0.57 (0.47–0.66) |
0.17 | 0.31 | 32 | 23 | 0.49 |
| MASP1 | 0.23 | 0.59 (0.49–0.69) |
0.06 | 0.11 | 30 | 25 | 0.54 (0.45–0.64) |
0.35 | 0.44 | 27 | 16 | 0.55 |
| MMP9 | 0.62 | 0.59 (0.49–0.68) |
0.08 | 0.11 | 38 | 23 | 0.63 (0.54–0.72) |
<0.01 ** | <0.05 * | 36 | 25 | 0.29 |
| MPO | 0.26 | 0.53 (0.44–0.62) |
0.56 | 0.56 | 14 | 11 | 0.52 (0.44–0.60) |
0.61 | 0.61 | 21 | 16 | 0.87 |
| PON3 | 0.81 | 0.60 (0.51–0.69) |
<0.05 * | 0.09 | 27 | 11 | 0.57 (0.48–0.67) |
0.13 | 0.31 | 23 | 16 | 0.35 |
| PRTN3 | 0.64 | 0.60 (0.51–0.69) |
<0.05 * | 0.09 | 13 | 9 | 0.66 (0.58–0.75) |
<0.01 ** | <0.01 ** | 41 | 16 | 0.15 |
| SPARC | 0.41 | 0.55 (0.45–0.64) |
0.34 | 0.38 | 18 | 11 | 0.66 (0.58–0.75) |
<0.01 ** | <0.01 ** | 45 | 25 | 0.14 |
| TR | 0.27 | 0.74 (0.65–0.82) |
<0.01 ** | <0.01 ** | 52 | 39 | 0.55 (0.45–0.65) |
0.34 | 0.44 | 32 | 25 | <0.05 * |
Abbreviations: AUC, area under the receiver operating curve; CRC, colorectal cancer; 95% CI, 95% confidence interval; LC/MRM-MS, liquid chromatography/multiple reaction monitoring–mass spectrometry; PEA, proximity extension assay; p-value, apparent p-values without any adjustments; p-value adj, p-value after adjustment for multiple testing by the Benjamini–Hochberg method; Se, sensitivity; Sp, specificity. Protein abbreviations: CDH5, cadherin 5; IGFBP2, insulin-like growth factor binding protein 2; MASP1, mannan binding lectin serine protease 1; MMP9, matrix metalloproteinase 9; MPO, myeloperoxidase; PON3, serum paraoxonase lactonase 3; PRTN3, myeloblastin; SPARC, secreted protein acidic and rich in cysteine; TR, transferrin receptor protein 1. Note: *, Significant at a p-value of < 0.05; **, significant at a p-value of < 0.01.