Table 5.
Diagnostic performance of 14 biomarkers overlapping between LC/MRM-MS and IpA measurements for detecting CRC cases from participants in screening colonoscopy.
| Marker | Pearson’s Correlation Coefficient |
LC/MRM-MS Measurements | IpA Measurements | DeLong p-value for AUC Testing |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AUC (95% CI) |
p-value | p-value adj | Se % at 80% Sp |
Se % at 90% Sp |
AUC (95% CI) |
p-value | p-value adj | Se % at 80% Sp |
Se % at 90% Sp |
|||
| A1BG | 0.04 | 0.66 (0.51–0.80) |
<0.05 * | 0.15 | 45 | 34 | 0.53 (0.38–0.69) |
0.69 | 0.80 | 17 | 10 | 0.26 |
| A2HS | 0.03 | 0.63 (0.48–0.78) |
0.10 | 0.28 | 45 | 38 | 0.54 (0.38–0.69) |
0.63 | 0.80 | 17 | 3 | 0.41 |
| C4BPA | −0.05 | 0.69 (0.55–0.83) |
<0.01 ** | <0.05 * | 48 | 45 | 0.55 (0.40–0.71) |
0.50 | 0.80 | 24 | 14 | <0.05 * |
| C6 | −0.13 | 0.66 (0.51–0.80) |
<0.05 * | 0.15 | 41 | 28 | 0.57 (0.42–0.73) |
0.34 | 0.80 | 15 | 7 | 0.44 |
| CA1 | 0.45 | 0.57 (0.42–0.72) |
0.37 | 0.58 | 24 | 17 | 0.57 (0.42–0.73) |
0.34 | 0.80 | 15 | 6 | 0.97 |
| CD5 | 0.00 | 0.57 (0.41–0.73) |
0.36 | 0.58 | 48 | 21 | 0.57 (0.42–0.72) |
0.36 | 0.80 | 15 | 7 | 0.99 |
| HSPA5 | 0.08 | 0.57 (0.42–0.73) |
0.80 | 0.94 | 24 | 17 | 0.52 (0.37–0.67) |
0.35 | 0.80 | 15 | 7 | 0.62 |
| IGHG1 | −0.21 | 0.55 (0.40–0.70) |
0.78 | 0.94 | 31 | 10 | 0.52 (0.37–0.67) |
0.55 | 0.80 | 13 | 5 | 0.82 |
| PLS2 | −0.03 | 0.54 (0.39–0.69) |
0.58 | 0.81 | 24 | 7 | 0.57 (0.41–0.72) |
0.40 | 0.80 | 15 | 7 | 0.84 |
| S100A9 | −0.02 | 0.57 (0.42–0.72) |
0.33 | 0.58 | 34 | 28 | 0.51 (0.36–0.66) |
0.88 | 0.94 | 12 | 6 | 0.57 |
| SAP | −0.03 | 0.72 (0.58–0.85) |
<0.01 ** | <0.05 * | 55 | 34 | 0.50 (0.35–0.66) |
0.96 | 0.96 | 12 | 5 | <0.05 * |
| SERPINF2 | −0.06 | 0.51 (0.36–0.66) |
0.90 | 0.94 | 28 | 17 | 0.55 (0.39–0.70) |
0.56 | 0.80 | 21 | 14 | 0.75 |
| SPARC | 0.00 | 0.62 (0.47–0.77) |
0.13 | 0.30 | 41 | 17 | 0.54 (0.39–0.69) |
0.63 | 0.80 | 17 | 8 | 0.47 |
| TR | −0.08 | 0.51 (0.35–0.66) |
0.94 | 0.94 | 24 | 21 | 0.59 (0.45–0.74) |
0.21 | 0.80 | 15 | 7 | 0.41 |
Abbreviations: AUC, area under the receiver operating curve; CRC, colorectal cancer; 95% CI, 95% confidence interval; IpA, immunome protein array; LC/MRM-MS, liquid chromatography/multiple reaction monitoring–mass spectrometry; p-value, apparent p-values without any adjustments; p-value adj, p-value after adjustment for multiple testing by the Benjamini–Hochberg method; Se, sensitivity; Sp, specificity. Protein abbreviations: A1BG, alpha-1B-glycoprotein; A2HS, alpha-2-HS-glycoprotein; C4BPA, C4b-binding protein alpha chain; C6, complement component C6; CA1, carbonic anhydrase 1; CD5, CD5 antigen; HSPA5, 78 kDa glucose-regulated protein; IGHG1, immunoglobulin gamma-1 chain C region; PLS2, plastin-2; S100A9, protein S100-A9; SAP, serum amyloid P-component; SERPINF2, alpha-2-antiplasmin; SPARC, secreted protein acidic and rich in cysteine; TR, transferrin receptor protein 1. Note: *, Significant at a p-value of < 0.05; **, significant at a p-value of < 0.01.