Figure 1.

Effects of elacridar on the barrier function of bEnd3 cells. (A) Exposing bEnd3 cells monolayers to elacridar resulted in the reduction of TEER measurements. TEER unit is in Ω * cm². (B) Elacridar significantly increased the permeability (Pc, cm/sec) of LY, a permeation marker, in a concentration and time-dependent manner. (C) Representative Western blot and densitometry analysis of P-gp and claudin-5 in bEnd3 cells, presented as fold change by elacridar compared to vehicle treatment, showed elacridar significantly reduced both proteins expression when compared to vehicle treated group. Statistical analysis was determined by one-way ANOVA test for (A,B) and Student’s t-test for (C). Data are presented as mean ± SD of at least three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001. kDa indicates the molecular weight of analyzed proteins.