Figure 3.

Treatment with elacridar (10 mg/kg) i.p. for 28 days disrupted BBB integrity in TgSwDI mice. (A) Representative Western blot and densitometry analysis of ZO-1 and claudin-5 in vivo from microvessels isolated from mouse brains. Elacridar treatment significantly decreased the expression of ZO1 in isolated microvessels from mouse brains when compared to the vehicle-treated group. kDa indicates the molecular weight of analyzed proteins. (B) Representative brain sections stained with anti-mouse IgG antibody to detect IgG extravasation (green) and anti-collagen antibody (red), with their optical density quantification in mouse brain hippocampus and cortex. White arrows indicate BBB leakage as demonstrated by IgG extravasation (green). Elacridar increased IgG extravasation in mouse hippocampus and cortex compared to vehicle-treated mice. Scale bar = 100 µm. Statistical analysis was determined by Student’s t-test. Data represented as mean ± SEM of n = 5 mice per group. ns = not significant; * p < 0.05, *** p < 0.001 compared to vehicle.