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. 2021 Jan 24;22(3):1140. doi: 10.3390/ijms22031140

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Alpl^−/− bone marrow stromal cells exhibit increased total colony forming units and adipocyte colony forming units. (A) The total number of crystal violet-stained colonies was significantly increased in Alpl^−/− compared to Alpl^+/+ BMSCs (bone marrow stromal cells). (B) The area of crystal violet stain per well significantly increased in Alpl^−/− compared to Alpl^+/+ BMSCs. (C) Representative images of crystal violet stain in Alpl^+/+ BMSCs and Alpl^−/− BMSCs. (D) The total number of colonies stained for alkaline phosphatase significantly decreased in Alpl^−/− compared to Alpl^+/+ BMSCs. (E) The area of alkaline phosphatase stain per well significantly decreased in Alpl^−/− BMSCs. (F) Representative images of alkaline phosphatase stain in Alpl^+/+ BMSCs and Alpl^−/− BMSCs. (G) The total number of colonies stained with oil red (for adipocytes) significantly increased in Alpl^−/− as compared to Alpl^+/+ BMSCs. (H) The area of oil red stain per well significantly increased in Alpl^−/− BMSCs. (I) Representative images of oil red stain in Alpl^+/+ BMSCs and Alpl^−/− BMSCs. n = 3 per genotype. * p < 0.05, statistical significance between genotypes. # = number.