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. 2021 Jan 24;22(3):1140. doi: 10.3390/ijms22031140

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TNAP deficiency increases osteoblastic and adipocytic differentiation in Alpl^−/− bone marrow stromal cells (BMSCs) as assessed by gene expression. The osteoblast genes, Bglap (A), Ibsp (B), Col1a1 (C), and Sp7 (D) significantly increased in the Alpl^−/− compared to Alpl^+/+ BMSCs, when cultured in non-differentiation media (no tx) or osteoblast differentiation media containing ascorbate (Asc) for 6 days. The adipocyte genes, Adipsin (E), Adipoq (F), Fabp4 (G), and Pparg (H) significantly increased in the Alpl^−/− compared to Alpl^+/+ BMSCs, when cultured in adipocyte induction then maintenance media (AdI/M) for 6 days total. Passage 2 cells were used. n = 3 per genotype. * p < 0.05, statistical significance between genotypes.