. 2021 Jan 24;22(3):1140. doi: 10.3390/ijms22031140

Table 1.

TNAP deficiency alters mitochondrial and non-mitochondrial metabolic activity.

Cell Type	Geno Type	Basal Respiration	ATP Production	Proton Leak	Maximal Respiration	Space Capacity	Non-Mito Oxygen Consumption
MC3T3E1 Cranial Osteoprogenitor Cell Line	NT shRNA	2.4±0.3	1.9±0.3	0.54±0.08	6.0±1.2	3.6±0.90	0.9±0.4
MC3T3E1 Cranial Osteoprogenitor Cell Line	Alpl shRNA	4.8±0.6 **	3.8±0.6 *	0.99±0.03 **	11.5±2.2 *	6.7±1.7	1.6±0.5
Primary Cranial Osteoprogenitor Cells	Alpl ^+/+	10.8±0.6	9.3±0.5	1.40±0.20	28.8±5.5	18.0±5.5	4.9±0.5
Primary Cranial Osteoprogenitor Cells	Alpl ^−/−	12.6±1.0 **	10.3±0.5 *	2.27±0.56 *	33.6±5.9	21.0±6.1	6.3±0.6 **
Primary Bone Marrow Stromal Cells	Alpl ^+/+	6.2±0.3	5.2±0.4	1.05±0.48	12.0±1.7	5.8±1.6	3.0±0.9
Primary Bone Marrow Stromal Cells	Alpl ^−/−	9.4±0.8 *	7.5±0.7 **	1.83±0.33	16.4±1.2 *	7.1±1.6	2.1±0.4
Sol8 Skeletal Muscle Cell Line	NT shRNA	33.9±4.6	27.1±3.7	6.83±1.63	72.1±13.5	38.2±10.0	11.9±4.3
Sol8 Skeletal Muscle Cell Line	Alpl shRNA	50.7±6.3 **	40.6±.8 **	10.14±1.58 **	152.5±21.2 ***	101.8±16.9 ***	18.4±2.7 *

Results shown are means ± standard error. n = 3 per genotype. * p < 0.05, ** p < 0.01, *** p < 0.005 statistical significance between genotypes. Non-Mito = non-mitochondrial; NT = non-target/control.