Table 3.
Primer and probe sequences in the expression analysis of selected candidate genes associated with tolerance to de-acclimation in winter barley.
| Gene | Forward Primer 3′-5′ | Reverse Primer 3′-5′ | Probe 3′-5′ |
|---|---|---|---|
| Peroxidase | GCACTTCCACGACTGCTTTG | CCATGCCAGACAGCAGAACA | FAM-CCAAGGTTGTGACGCGT-MGB |
| Catalase | GGACCTGCTCGGCAACAA | GGGCTTGAAGGCGTGGAT | FAM-CCCCGTCTTCTTCA-MGB |
| CBF14 | CAGCATCCATCTCTCCCAAGTC | TGTGGAGTAAGCAGCGTGTTTT | FAM-CAGCGCAGCAGCT-MGB |
| PGU inhibitor-like | TACCACTTTGCGTCCTGGAC | TCAGCATCACAGTCGACGTC | FAM-GCCCGACTCCGCCTGTTGC-MGB |
| sHSP | GTCGCCATCGCCTGATCT | TGACAAACGCCGATGAGGTA | FAM-TACCTCAGTCGCGCCAG-MGB |