Figure 3.

Composition of the 28 hypoxia gene signatures to prognosticate localized PCa and exemplify derivation of prognostic signatures by combining multiple sub-signatures [40]. The hypoxic component was comprised by (1) a 25-gene signature identified through TaqMan Low Density Array-Hypoxia Scores on tumor samples from 80 head and neck squamous cell carcinoma (HNSCC) patients [92], (2) a metagene enriched in hypoxia-regulated pathways through in vivo co-expression pattern data from head, neck, and breast cancer studies [93], (3) studies linking Myc to the modulation of the hypoxic response [94], (4) a 32-gene signature positively correlating to Ki-67 staining that was shown to link the hypoxia marker pimonidazole and aggressive PCa [95], (5) a 15 gene hypoxia-related expression classifier from in vivo xenograft tumors validated in 323 HNSCC patients [96], and (6) a metagene obtained by analysis of genes whose in vivo expression clustered with the expression of 10 well-known hypoxia-regulated genes [97]. Created with BioRender.com.