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. 2021 Jan 27;13(3):483. doi: 10.3390/cancers13030483

Figure 1.

Figure 1

Transcription factor EB (TFEB) knockdown reduces the pancreatic ductal adenocarcinoma (PDAC) growth. (A) Box plots derived from the gene expression data in Oncomine comparing the expression of the TFEB gene in six normal tissue samples (left plot) and in 11 pancreatic cancer samples (right plot). (B) TFEB gene expression was detectable in 179 pancreatic cancer tissues (T) and 171 normal tissues (N) from the gene expression profiling interactive analysis (GEPIA) database. (C) Immunoblots of the TFEB expression in human pancreatic ductal (HPDE) and PDAC cells. (D,E) Cell growth assay for PDAC cells infected with shRNAs against GFP or TFEB. Western blot analysis confirmed the knockdown of TFEB expression. (F,G) Cell viability assay for PDAC cells infected with shRNAs against GFP or TFEB. (H,I) Clonogenic assay for PDAC cells infected with shRNAs against GFP or TFEB. Error bars represent the standard deviation (SD) of triplicate wells from a representative experiment; * p < 0.05; ** p < 0.01. (Please find western blot in Supplementary files)