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. 2021 Jan 27;13(3):483. doi: 10.3390/cancers13030483

Figure 2.

Figure 2

Effects of TFEB on the basal levels of autophagy. (A) PDAC cells infected with shRNAs against GFP or TFEB were immunoblotted with the indicated antibodies. (B) PDAC cells expressing the control shRNA (shGFP) or shRNAs targeting TFEB were infected with a lentivirus expressing GFP-LC3 and analyzed for LC3 dots. (C,D) PDAC cells infected with shRNAs against GFP or TFEB were plated in compete media, which was replaced the following day with glutamine-free media. (C) The cells were immunoblotted with the indicated antibodies 48 h after supplementation with glutamine free media. (D) The cells were infected with a lentivirus expressing GFP-LC3 and analyzed for LC3 dots 48 h after supplementation with glutamine free media. ** p < 0.01. (Please find western blot in Supplementary files)