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. 2021 Jan 27;13(3):483. doi: 10.3390/cancers13030483

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TFEB inhibits mitochondrial metabolism. (A) Glycolysis metabolite pools in 8988T cells infected with shRNAs against GFP or TFEB were analyzed via LC-MS/MS. (B) The 8988T cells infected with shRNAs against GFP or TFEB were analyzed for intracellular adenosine triphosphate (ATP). (C) Oxygen consumption rates in 8988T cells infected with shRNAs against GFP or TFEB were measured with an extracellular flux analyzer. (D) TCA (tricarboxylic acid cycle) metabolite pools in 8988T cells infected with shRNAs against GFP or TFEB were analyzed via LC-MS/MS. Error bars represent the SD of triplicate wells from a representative experiment; * p < 0.05; ** p < 0.01.