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TFEB knockdown reduces the glutamate levels. (A) Glutamine levels in 8988T cells infected with shRNAs against GFP or TFEB were monitored using LC-MS/MS. (B) Relative clonogenic growth of 8988T cells infected with shRNAs against GFP or TFEB was analyzed by plating the cells in complete media supplemented with either glutamine (4 mM) or glutamate (4 mM). Error bars represent the SD of triplicate wells from a representative experiment; ** p < 0.01. Gln: Glutamine; Glu: Glutamate.
