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. 2021 Jan 28;22(3):1321. doi: 10.3390/ijms22031321

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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LUT-7G treatment in endothelial cells. (A) immunofluorescence analysis with Ki67 staining (green) of HUVEC cells in proliferating condition. (B) After 48 h without treatment. (C) After 48 h and LUT-7G treatment. (D–F) Same time course and treatment with vWF staining (green) (bars = 60 μm). (G,H) Higher enlargement of vWF staining, showing a cytoplasmic location in untreated cells (G) and a membrane staining in treated cells. Indeed, treated cells show an important reduction of vWF synthesis. In the picture, phalloidin is stained in red, and nuclei in blue (DAPI (4′,6-diamidino-2-phenylindole); bars = 60 μm; Prolif = proliferating endothelial cells).

LUT-7G treatment in endothelial cells. (A) immunofluorescence analysis with Ki67 staining (green) of HUVEC cells in proliferating condition. (B) After 48 h without treatment. (C) After 48 h and LUT-7G treatment. (D–F) Same time course and treatment with vWF staining (green) (bars = 60 μm). (G,H) Higher enlargement of vWF staining, showing a cytoplasmic location in untreated cells (G) and a membrane staining in treated cells. Indeed, treated cells show an important reduction of vWF synthesis. In the picture, phalloidin is stained in red, and nuclei in blue (DAPI (4′,6-diamidino-2-phenylindole); bars = 60 μm; Prolif = proliferating endothelial cells).