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Low magnesium induces EDF-1. HUVEC were cultured in medium containing 0.1, 0.3, 0.5, 1 or 5 mM of extracellular Mg for 24 h. Cell extracts were processed for Western blot using antibodies against EDF-1. Actin was used as a control of loading. A representative blot is shown. Densitometry (right panel) was performed on three different blots using Image J. ** p ≤ 0.01.
