Figure 2.

Low magnesium induces ROS accumulation. ROS production was evaluated using DCFDA in HUVEC maintained for 24 h in media containing different concentrations of Mg in the presence (orange columns) or in the absence (green columns) of NAC (5 mM). Fluorescence at 529 nm was measured and then normalized to the cell number. The experiment was performed three times in triplicates. * p ≤ 0.05 and ** p ≤ 0.01.