BLI	Biolayer interferometry
CD	Circular dichroism
EDC	3-(Ethyliminomethyleneamino)-N, N-dimethylpropan-1-amine
EDTA	Ethylenediaminetetraacetic acid
ESI-TOF-MS	Electrospray ionization time-of-flight mass spectrometry
FRET	Fluorescence resonance energy transfer
HPLC	High-performance liquid chromatography
KD	Affinity dissociation constant
MALDI	Matrix-assisted laser desorption/ionization
MST	Microscale thermophoresis
NHS	N-Hydroxysuccinimide
NMR	Nuclear magnetic resonance
PAGE	Polyacrylamide gel electrophoresis
SAX	High-sensitivity streptavidin-activated biosensors
SELEX	Systematic Evolution of Ligands by Exponential Enrichment
SPR	Surface plasmon resonance
SPRi	Surface plasmon resonance imaging
T15	15× thymine spacer
TBA	Thrombin binding aptamer
TBE	Tris-borate/EDTA
TEG	Triethylene glycol
UV	Ultraviolet