Table 1.
Primer sequences for qPCR.
| Gene * | Primer Sequence (5′–3′) | Size (bp) | Accession No. |
|---|---|---|---|
| IL-1β | Forward: GAAAGATAACACGCCCACCC Reverse: TCTGCTTGAGAGGTGCTGATGT |
165 | NM_214055.1 |
| IL-6 | Forward: CCTGTCCACTGGGCACATAAC Reverse: CAAGAAACACCTGGCTCTGAAAC |
252 | NM_214399.1 |
| TNF-α | Forward: CATCGCCGTCTCCTACCA Reverse: CCCAGATTCAGCAAAGTCCA |
199 | NM_214022.1 |
| IFN-γ | Forward: GAGCCAAATTGTCTCCTTCTAC Reverse: CGAAGTCATTCAGTTTCCCAG |
140 | NM_213948.1 |
| Bcl-2 | Forward: AGCATGCGGCCTCTATTTGA Reverse: GGCCCGTGGACTTCACTTAT |
120 | XM_021099593.1 |
| Bcl-xL | Forward: GGTCGCATTGTGGCCTTTTT Reverse: CGTCAGGAACCATCGGTTGA |
237 | NM_214285.1 |
| Bax | Forward: CTGACGGCAACTTCAACTGG Reverse: CGTCCCAAAGTAGGAGAGGA |
200 | XM_003127290.5 |
| TNFR1 | Forward: CTGGCATTCTTCCTCTTCGTTG Reverse: CCGGCTCTCCCTCCTTTACA |
109 | NM_213969.1 |
| TRADD | Forward: AGGCGTGCTTGGAGGCT Reverse: GCGAAGATGAAATTCAAACAGC |
124 | XM_021094047.1 |
| FADD | Forward: CTGCGACAACGTGGGGA Reverse: TCAGGTTTCGGGGATACTTC |
101 | NM_001031797.1 |
| GAPDH | Forward: ATGGTGAAGGTCGGAGTGAAC Reverse: CTCGCTCCTGGAAGATGGT |
235 | NM_001206359.1 |
*IL-1β: interleukin-1β. IL-6: interleukin-6. TNF-α: tumour necrosis factor-α. IFN-γ: interferon-γ. Bcl-2: B-cell lymphoma-2. Bcl-xL: Bcl-2-like-1. Bax: B-cell lymphoma-2-associated X protein. TNFR1: tumour necrosis factor receptor 1; TRADD: tumour necrosis factor receptor-associated death domain. FADD: Fas-associated death domain. GAPDH: glyceraldehyde-3-phosphate dehydrogenase.