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. 2021 Jan 28;22(3):1303. doi: 10.3390/ijms22031303

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Altered expression of flowering genes in the rfs-2 mutant during plant development. Total RNA was isolated from HY and rfs-2 leaf blades collected 1 h after dawn (ZT1) in SD (a,c,e) and LD (b,d,f) conditions. Transcript levels of Hd3a (a,b), RFT1 (c,d), and Ehd1 (e,f) were determined by RT-qPCR and normalized to that of OsUBQ5 (LOC_Os01g22490). Means and standard deviations were obtained from three biological replicates. Experiments were repeated three times with similar results. Red arrows indicate the average flowering date of HY.