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. 2021 Jan 22;22(3):1099. doi: 10.3390/ijms22031099

Figure 1.

Figure 1

Figure 1

Evaluating the effect of the cyclic RGDfK-liposome on αVβ3 integrin-mediated cell adhesion. (A) The cyclic RGDfK-liposome preparation was showed. A375.S2 cells was pretreated with 100 nM cRGDfV, cRGDfK, cRGDfK-PEG2000-DSPE or the cRGDfK-liposome and allowed to adhere to a coating of fibronectin, collagenI or collagen IV for 1 h at 37 °C before washing and detection of cell adhesion (B) or followed by staining with FITC-labeled mouse anti-human CD51/CD61 monoclonal antibody (C). The black star indicated p < 0.01 and duplicate black stars for p < 0.05, when compared with liposome group; N.S., non-significant. These experiments were performed for five times.