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. 2021 Jan 22;22(3):1099. doi: 10.3390/ijms22031099

Figure 4.

Figure 4

Figure 4

Evaluating the effect of the cyclic RGDfK-liposome on functioning of phagocytes. (A) Determining the optimal ratio of RAW 264.7 cells to the opsonized fluorescent-labeled Escherichia coli (E. coli). (B) The measurement of the fluorescent-labeled Escherichia coli (E. coli) engulfed in RAW264.7 cells in the presence of liposome (panels a–c) or the cyclic RGDfK-liposome (panels d–f). (C) The measurement of reactive oxygen species generation in RAW264.7 cells in the presence of liposome (panel a) or the cyclic RGDfK-liposome (panel b) (D) Measuring the effect of liposome or the cyclic RGDfK-liposome on reactive oxygen species generation in RAW264.7 cells with low concentration (panel a) or high concentration (panel b) in the presence of lipopolysaccharide (LPS). These experiments were performed five times.