Figure 15.

Intracellular fluorescent OFF-ON detection of AIB1 protein in MCF-7 breast cancer cells using US-propelled aptamer (FAM-AIB1-apt) functionalized nanomotors. (A) Schematic illustration of OFF-ON strategy based on fluorescence switching. Fluorescence of FAM-AIB1-apt is quenched due to adsorption at GO surface. Subsequently, the fluorescence signal is recovered in presence of specific target AIB 1. (B) Representative microscopic optical, fluorescence and merged images corresponding to cells treated under different conditions (a–e rows). (C) Bar plot displaying the fluorescence intensity obtained under different treatments (a–e) describe in (B). Ultrasound field parameters: voltage amplitude 2 V, resonant frequency 2.66 MHz, and 15 min. Scale bar 50 μm. Reproduced from [17] with permission of Wiley-VCH.