Figure 11.

ROS production in HOG (a) and SH-SY5Y cells (b) treated with increasing concentrations (50–100 nM) of hApo D for 24 h followed by 24 h with 500 or 1000 µM of CPZ. Changes in ROS levels, measured with the oxidant-sensitive dye H₂DCFDA, are represented as the percentage of fluorescent DCF production versus control. H₂O₂ 500 µM was used as positive control. Data are the mean ± SEM of five independent experiments. Significant differences were analyzed by a one-way ANOVA followed by post-hoc Tukey’s test. *** p < 0.001 compared with control.