Figure 4.

Allium fistulosum cytotoxic effect on BJ cells with 40× objective ((A)—Control group, (B)—0.5% extract, (C)—IC₅₀ dose, and (D)—10% extract) and HaCaT cells with 100× objective ((E)—control group, (F)—0.1% extract, (G)—IC₅₀ dose, and (H)—10% extract). Cells were labeled with different strainers, DAPI for the nucleus (blue), Mitotracker for the mitochondria (red), and Phalloidin-FITC for cytoskeleton (green). Nuclear damage—White arrows; Cytoskeleton disruption—Red arrows; Mitochondrial network reduced intensity—Yellow arrows; Mitochondrial intensified activity—Turquoise arrows.