Figure 9.

MTT assay for DLD-1, MDA-MB-231, MCF-7, and SK-MES-1 cell lines treated with different concentrations of Allium sativum extract. The results were expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001 with Student’s t-test by comparison with control group (untreated). The samples treated with 10% extract in the culture medium (equivalent to 100 mg mL⁻¹) showed highly significant inhibition, with a significant inhibitory effect even at 1% extract. SK-MES-1 was the most sensitive cell line with 4.651% extract as the IC₅₀ value (equivalent to 46 mg/mL) and MDA-MB-231 the triple negative breast cancer cell line being the less sensitive cell line with 6.375% extract (equivalent to 63 mg/mL) as the IC₅₀ value.