Table 1.
Elution time, analytical method characteristics, and determined concentrations of alliin, allicin, and phenolic acids and flavonoids in the Allium extract samples (results expressed as the mean value of 4 analyzed samples, n = 4).
| No | Compounds | telution (min) | R2 | LOD (µg/mL) |
A. sativum (µg/mL) * |
A. fistulosum (µg/mL) |
|---|---|---|---|---|---|---|
| 1 | Alliin | 3.77 | 0.9999 | 5.8 | 1410 ± 50 | 145 ± 15 |
| 2 | Allicin | 15.40 | 0.9999 | 14.1 | 380 ± 15 | 20 ± 5 |
| 3 | Gentisic ac. | 8.13 | 0.9997 | 3.4 | 60 ± 5 | - |
| 4 | Chlorogenic ac. | 9.15 | 0.9995 | 4.6 | 65 ± 5 | - |
| 5 | 4-hydroxybenzoic | 10.31 | 0.9999 | 2.3 | 25 ± 3 | - |
| 6 | Rutin | 11.39 | 0.9998 | 2.7 | - | 215 ± 3 |
| 7 | Isoquercitrin | 11.91 | 0.9999 | 2.2 | - | 280 ± 3 |
| 8 | p-Coumaric | 12.38 | 0.9999 | 1.9 | 44 ± 4 | - |
| 9 | Quercitrin | 12.72 | 0.9998 | 2.7 | - | 95 ± 3 |
| 10 | Ferulic ac. | 12.85 | 0.9999 | 2.0 | - | 230 ± 2 |
| 11 | Quercetin | 16.35 | 0.9997 | 3.3 | - | 26 ± 3 |
| 12 | Kaempferol | 19.77 | 0.9998 | 2.7 | - | 30 ± 3 |
LOD—limit of detection, R2—coefficient of determination for the calibration curves (at six levels of concentrations). Indicated intervals represent the average ± standard deviations (n = 4). * The information regarding Allium sativum was previously published by Pârvu et al. [10].