Figure 1.

Effects of fixation time on polyphosphate detection in platelets. Platelets suspended in phosphate-buffered saline were immobilized on glass slides using a Cytospin and fixed in 10% neutral-buffered formalin for (a) 1 h, (b) 2 h, (c) 4 h, or (d) 18 h. Platelets were then treated with 4′,6-diamidino-2-phenylindole (DAPI; green) and phalloidin (red) in 0.1% Tween-20-containing Hanks balanced salt solution and examined using a fluorescence microscope. (e) Quantification of DAPI staining in the fluorescence images (above). A significant difference was obtained via a parametric test but not by using a non-parametric test.